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Mueller Hinton Agar, 500gm Hi-Media
Price :
৳12500
Estimated Shipping Time: Deliveries take up to 2-3 days after you place your order.
Uses: Lab Essentials
Product SKU: DYo3156YN9
Mueller and Hinton developed Mueller Hinton Agar (MHA) in 1941 for the isolation of pathogenic Neisseria species. Nowadays, it is more commonly used for the routine susceptibility testing of non-fastidious microorganism by the Kirby-Bauer disk diffusion technique.
Composition of MHA :
| Ingredients | In Gram/Litre |
|---|---|
| Beef Extract | 2.00 gm |
| Acid Hydrolysate of Casein | 17.50 gm |
| Starch | 1.50 gm |
| Agar | 17.00 gm |
| Distilled Water | 1000 ml |
Uses of MHA:
- The major use of Mueller Hinton Agar is for antimicrobial susceptibility testing. It has become the standard medium for the Bauer Kirby method and its performance is specified by the NCCLS.
- It can be used to cultivate Neisseria
- It is specified in FDA Bacteriological Analytical Manual for food testing, and procedures commonly performed on aerobic and facultative anaerobic bacteria.
Preparation of MHA :
- Suspend 38 gm of the medium in one liter of distilled water.
- Heat with frequent agitation and boil for one minute to completely dissolve the medium.
- Autoclave at 121°C for 15 minutes. Cool to room temperature.
- Pour cooled Mueller Hinton Agar into sterile petri dishes on a level, horizontal surface to give uniform depth.
- Allow to cool to room temperature.
- Check for the final pH 7.3 ± 0.1 at 25ºC.
- Store the plates at 2-8 ºC.
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- Mueller Hinton Agar M173
- Intended Use:
- Recommended for determination of susceptibility of microorganisms to antimicrobial agents isolated from clinical samples.
- Composition**
- Ingredients Gms / Litre
- HM infusion B from # 300.000
- Acicase ## 17.500
- Starch 1.500
- Agar 17.000
- Final pH ( at 25°C) 7.3±0.1
- Mueller Hinton Agar
- Pack Size: 500gm
- Brand: Hi-Media
- Origin: India
**Formula adjusted, standardized to suit performance parameters.
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