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Brilliant Green Bile Broth 2%, 500gm
Price :
৳8000
Size :
- 500gm
Color :
Estimated Shipping Time: Deliveries take up to 4-5 days after you place your order
Uses: Laboratory and scientific purposes
Product SKU: kkb0515kSO
- Brilliant Green Bile Broth 2%
- Brand: Hi-Media
- Part Number: M121-500G
- Pack Size: 500gm
- Made in India
Application :
1. Selective Growth Medium: Brilliant green can inhibit the growth of certain bacteria, making the medium selective for specific microorganisms. This is useful when trying to isolate and identify particular bacterial strains.2. Identification of Enteric Bacteria: Brilliant Green Bile Broth is commonly used in the identification of enteric bacteria, particularly those belonging to the Enterobacteriaceae family. It is often used in conjunction with other differential media to differentiate between different types of bacteria based on their ability to ferment lactose.3. Detection of Coliforms: The medium is often used for the detection of coliform bacteria, which are indicators of fecal contamination. Coliforms can ferment lactose, producing acid and gas, which can be detected in the medium.4. Microbiological Testing: Brilliant Green Bile Broth can be employed in various microbiological tests to assess the presence and characteristics of specific microorganisms.
Contact
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Brilliant Green Bile Broth 2% is a microbiological medium commonly used for the detection and confirmation of coliform bacteria, especially in water and food samples. Here are 10 key points related to Brilliant Green Bile Broth 2%:
Purpose: The primary purpose of Brilliant Green Bile Broth 2% is the selective enrichment of coliform bacteria from samples, allowing for their subsequent detection and identification.
Selective Agents: It contains bile salts and Brilliant Green dye as selective agents. Bile salts inhibit the growth of Gram-positive bacteria, while Brilliant Green inhibits the growth of most Gram-negative bacteria other than coliforms.
Coliform Indicator: Coliform bacteria, including Escherichia coli (E. coli), ferment lactose, producing gas and acid. The medium is designed to support the growth of these coliforms and detect their presence.
Lactose Fermentation: The medium contains lactose as a fermentable carbohydrate. Coliform bacteria that ferment lactose produce acid and gas during the process.
Indicator of Fecal Contamination: Detection of coliforms in water or food samples using Brilliant Green Bile Broth is an indicator of possible fecal contamination, suggesting the presence of potentially harmful microorganisms.
Presumptive Test: Brilliant Green Bile Broth is often used as a presumptive test in the detection of coliforms before confirmation on selective agar plates such as Eosin Methylene Blue (EMB) agar or MacConkey agar.
Double Strength: The "2%" in Brilliant Green Bile Broth 2% indicates that it is double-strength, which means it contains twice the concentration of typical ingredients compared to a standard broth.
Incubation Conditions: Incubation is typically carried out at 35-37°C for 24-48 hours. This allows for the growth and acid/gas production by coliform bacteria.
Appearance Changes: Acid production from lactose fermentation can cause a color change in the medium. The broth may turn yellow due to the accumulation of acid.
Quality Control: Like all laboratory media, Brilliant Green Bile Broth 2% undergoes quality control measures to ensure consistency and reliability in performance. Proper storage and adherence to protocols are crucial for accurate results.
Laboratories use Brilliant Green Bile Broth 2% as part of their testing procedures for water quality, food safety, and environmental monitoring, providing valuable information about the presence of coliform bacteria in different samples.
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