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Dichloran Rose Bengal Chloramphenicol (DRBC) Agar 500gm
Price :
৳11500
Estimated Shipping Time: Deliveries take up to 2-3 days after you place your order.
Uses: Lab Essentials
Product SKU: Jqv19337Pi
- DRBC Agar (Dichloran Rose Bengal Chloramphenicol Agar)
- Pack Size: 500gm
- Brand: Himedia
- Made in India
Applications:
- For enumeration of fungal species found in food samples.
- Selective isolation of yeasts and molds from food and soil samples.
Advantages Of DRBC Medium:
- It prevents bacterial growth to some extent due to low pH and chloramphenicol.
- Some fungal and bacterial species take up rose-bengal (bacterial colonies can be observed in plates lacking chloramphenicol). This makes colony counting easier.
PH :
Although the medium can be used at pH 7.2, a pH ranging between 5.40 − 5.80 (average 5.6) is recommended to inhibit bacterial growth. A higher pH, like 7.2, not only increases the risk of bacterial contamination, but also leads to faster growth and larger colonies of fungi.
Preparation :
- Add required quantities of all the components except for agar and chloramphenicol in 800 mL of distilled water.
- Adjust the pH and make up the volume to 1L.
- Aliquot the solution if necessary.
- Add agar.
- Sterilize by autoclaving at 121°C for 20-30 minutes.
- Add chloramphenicol when the medium has sufficiently cooled down.
- Pour plates. Seal with parafilm.
Storage :
- Wrap bottles containing DRBC medium with aluminum foil to avoid exposure to light.
- DRBC medium can be stored at 4°C for a maximum of 4 weeks.
- Use freshly poured plates within 2 weeks.
- If plates are made by reheating stored DRBC agar, use them within a week.
If you'd like to know more about this chemical or need any analysis report regarding this chemical then contact us support@echem.com.bd.
Composition of DRBC agar
| Component | For 1L media (in grams) |
|---|---|
| Peptone | 5g |
| Dextrose (Glucose) | 10g |
| Potassium dihydrogen phosphate | 1g |
| Magnesium sulphate | 0.5g or 500mg |
| Rose Bengal | 0.025g or 25mg |
| Chloramphenicol | 0.100g or 100mg |
| Dichloran | 0.002g or 2mg |
| Agar | 15g |
Points To Be Noted :
- Add agar after adjusting the pH of the medium to avoid hydrolysis of agar.
- Protect the liquid medium and plates from light. Rose-bengal is light-sensitive, and its photodegradation product is toxic to fungi.
- Do not use the medium which is past its recommended storage time as the colony count can be affected.
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