Mueller Hinton Agar, 500gm Hi-Media for lab grade
Mueller Hinton Agar is primarily used for antimicrobial susceptibility testing.It provides a standardized medium for the Kirby-Bauer disk diffusion method.It supports the growth of a wide range of microorganisms.The agar's low content of cations (Mg2+ and Ca2+) minimizes their interference with antimicrobial agents.Preparation of MHA :
- Suspend 38 gm of the medium in one liter of distilled water..
- Autoclave at 121°C for 15 minutes. Cool to room temperature.
- surface to give uniform depth.
- Allow to cool to room temperature.
- Check for the final pH 7.3 ± 0.1 at 25ºC.
- Store the plates at 2-8 ºC
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