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Leishman Stain 500mL
Price :
৳550
Size :
- 500 ml
Estimated Shipping Time: Deliveries take up to 3-5 days after you place your order.
Uses: Lab Essentials
Product SKU: khZ0714Ac1
Leishman Stain 500mL
Leishman stain is a type of Romanowsky stain used primarily in histology and cytology to visualize blood cells and certain microorganisms. It is beneficial for identifying parasites like Leishmania, which causes leishmaniasis, and assessing the morphology of various blood cell types.
- Applications of Leishman Stain Solution:
- Parasitology: Identifying Leishmania in smears from skin lesions or blood.
- Hematology: Assessing blood smears for various conditions, including infections and blood disorders.
- Procedure: The staining process typically involves fixing a specimen (like a blood smear), applying the stain for a specific duration, and then rinsing to reveal the stained components under a microscope.
- Visualization: The stain highlights different cellular elements, such as:
- Nuclei (which appear blue)
- Cytoplasm (which can take on various shades depending on the cell type)
- Parasites may be visualized as distinct structures within the cells.
- Advantages:
- Simple and cost-effective.
- Provides clear differentiation of cellular components, aiding in diagnosis.
- Limitations: Requires careful preparation of smears and may not be suitable for all types of microorganisms or tissue samples.
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Leishman Stain 500mL
v Composition:
- Methylene Blue: 0.15–0.18 g/dL – provides blue color, staining the cell nuclei and basophilic cell components.
- Eosin Y: 0.15–0.18 g/dL – provides pink or red color, staining the cytoplasm and acidophilic components.
- Methanol: Serves as both solvent and fixative, allowing the stain to penetrate cells effectively.
v Concentration:
- The solution is typically prepared with approximately 0.15–0.18% dye concentration in methanol.
v pH:
- Optimal pH range: 6.8–7.2 – to ensure proper staining characteristics, as deviations can lead to poor differentiation of cellular components.
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